Main Products:Bacillus subtilis,Bacillus licheniformis,Alkali Protease,Neutral Protease,Medium Temperature α-amylase
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Mid-Temperature α-Amylase

Contact:Linda Lee




I. Brief Introduction:

Alpha-Amylase is made of Bacillus Subtilis in the Fermentation and extraction method. It is widely used in the liquefaction of starch sugar, alcohol, beer, monosodium glutamate, animal feed, Fermentation industries and textile, printing & dyeing, de-sizing and etc.

II. Definition of Activity:

One gram of solid alpha-amylase powder (or one milliliter of liquid alpha-amylase) at 60°C and pH6 liquefies 1 mg of starch in one hour is defined as one enzyme activity unit and marked with u/g or u/ml.

III. Characteristics:

1. Heat stability: Stable at 60°C or below; Optimum operational temperature 60 -70°C; Applicable to the liquefaction process of 90°C (highest).

2. pH Stability: More stable at pH6.0-7.0; Optimum operational pH below pH6.0;

3. Ca+: required.

IV. Specification:

Attribute Name

Attribute Value


Yellow-Brown( White)Powder/ Brown liquid

Enzyme Activity

2000 u/g~10,000 u/g, 2000 u/ml~ 5000u/ml

Particle Size (Powder Type) 

≥90% pass 40 mesh

Loss on Drying(Powder Type)


Volume Weight(Liquid Type)

≤1.20 g/ml


GB/T24401-2009; QB1805.1-93.

V. Usage and Reference Dosage:

(The following dosage is an example of 2000 u/g, other specification can be converted according to the enzyme activity)

1. In beer brewing and alcohol industry: 

Reference dosage: 3.0L/Ton of raw materials, liquefying at 80-90°C and keep for 30 min.

2. In Starch Sugar, Maltose, Monosodium Glutamate and other fermentation Industry: 

Reference dosage: add 0.2% Calcium chloride(based on the weight of raw materials) and add amylase 3.0-4.0L/Ton of raw materials, liquefying at 80-90°C and keep for 30 min.

3. In Textile Desizing Industry: 

Reference dosage: 0.2% (o.w.f),  keep at 50-80°C for 20-40 min. 

(It is suitable for the material which can not withstand high temperature such as the silk, chemical fiber, woven fabric of cotton, wool and etc.)

4. In Feed Industry: 

Functions: Improving animals digestion, adjusting animals immunity and improving feed utilization.

Reference dosage: 0.02-0.04kg/Ton of complete formula feed. 

Generally, it is used with pectinase, β-Glucanase and Cellulase for better performance.

5. In juice industry: 

Functions: Improving the juice transparency and avoid turbidity.

Reference dosage: 0.02-0.1 L/Ton of juice raw materials and keep at 45°C for 60-120 min. 

Generally, it is used with Pectinase and Cellulase for better performance.

6. In steamed bread, bread and other flour products: 

Functions: Improving the product quality and lengthen the shelf life.

Reference dosage: 0.05-0.1kg/Ton of raw materials.

7. Other Industries:  

Reference dosage: 2-4 L/Ton of dry basis

Concentration of calcium ion:150ppm.

VI. Storage:

Should be stored in a cool, dry and well-ventilated place; protect from direct sunlight, heat and dampness; avoid storing with poisonous and harmful substances. 

VII. Packaging:

20kg/bag, 25 kg/bag ,25kg/drum, 30 kg/drum or as clients’ requirements.

VIII. Shelf Life:

Powder Type: Enzyme activity remains ≥ 90% after 12 months at room temperature 25°C.

Liquid Type: Enzyme activity remains ≥ 90% after 6 months at room temperature 25°C.

IX. Technical Support and Service:

Xingtai Sinobest Biotech Co., Ltd. will provide technical support service in the whole process. Our professional technicians will guarantee every client to use this product under proper guide.

X. Precautions:

Enzyme preparations belong to protein, which may induce sensitization and cause allergic type reactions in sensitized individuals. Prolonged contact may cause minor irritation for skin, eyes or mucous membrane of nose, so any direct contiguity with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.


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